The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis

Mitochondrial DNA (mtDNA), as a model sys-tem, has been extensively used for molecular phy-logenetic and evolutionary analysis[1]. With the ad-vances in DNA sequencing technology, more andmore researchers prefer to use complete mitochondrialgenome for phylogenetic analysis[2—4]. Since the com-plete sequencing of human mtDNA in 1981 (Andersonet al., 1981)[5], 342 vertebrate mitochondrial genomeshave so far been sequenced. Up to now the completesequences of 29 avian mitochondrial genomes h…     

Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells

~~Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells1. Jin, Q., Yuan, Z., Xu, J., Wang, Y., Shen, Y., Lu, W., Wang, J., Liu, H., Yang, J., Yang, P., Zhang, X., Zhang, J., Yang, G, Wu, H., Qu, D., Dong, J., Sun, L., Xue, Y, Zhao, A., Gao, Y., Zhu, J., Kan, B., Ding, K.. Chen, S., Cheng, H., Yao, Z., He, B., Chen, R., Ma, D., Qiang, B., Wen, Y, Hou, Y., Yu, J., Genome sequence of Shigella flexneri 2…     

Antitumor effects of the molecule-downsized immunoconjugate composed of lidamycin and Fab′fragment of monoclonal antibody directed against type IV collagenase

Type IV collagenase plays an important role in tumor invasion and metastasis through cleaving type IV collagen in the basement membrane and extracellular matrix. In this study a molecule-downsized immunoconjugate (Fab'-LDM) was constructed by linking lidamycin (LDM), a highly potent antitumor antibiotic, to the Fab' fragment of a monoclonal antibody directed against type IV collagenase and its antitumor effect was investigated. As assayed in 10% SDS-PAGE gel, the molecular weight of Fab'-LDM conjugate was 65 kD with a 1 : 1 molecular ratio of Fab' and LDM. The Fab'-LDM conjugate maintained most part of the immunoreactivity of Fab' fragment to both type IV collagense and mouse hepatoma 22 cells by ELISA. By MTT assay, Fab'-LDM conjugate showed more potent cytotoxicity to hepatoma 22 cells than that of LDM. Administered intravenously, Fab'-LDM conjugate proved to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings. In Experiment     

用4个微卫星标记分析7个绵羊群体之间的遗传关系

分析了4个微卫星基因座BM143、OarHH35、OarAE101、BMS2508在7个绵羊群体（小尾寒羊、湖羊、乌珠穆沁羊、萨福克羊、多赛特羊、夏洛来羊、多赛特公羊×小尾寒羊母羊F1代杂种羊）286只绵羊中的遗传多态性。结果表明,这4个微卫星标记在7个绵羊群体中的等位基因数分别为9、11、14和9,其多态信息含量/有效等位基因数/杂合度分别为0.7073/3.7231/0.7314、0.8267/6.4399/0.8447、0.5743/2.5178/0.6028、0.6172/3.0712/0.6744,其中OarHH35的遗传变异最大,OarAE101最小。7个绵羊群体中小尾寒羊的遗传变异最大,湖羊的最小。基于Nei氏DA距离和DS标准遗传距离,采用UPGMA方法构建了系统发生树。该发生树将中国地方品种（小尾寒羊、乌珠穆沁羊、湖羊）和法国的夏洛来羊归为一类,将F1杂种羊、英国品种（萨福克羊和多赛特羊）归为另一类。绵羊微卫星基因分型技术为检查品种（群体）之间的遗传关系提供了一个有用的工具。 Abstract：The genetic polymorphisms of four microsatellite loci BM143,OarHH35,OarAE101,and BMS2508 were analyzed in 286 sheep of seven sheep populations (Small Tail Han sheep, Hu sheep, Ujumqin sheep, Suffolk sheep, Dorset sheep, Charolais sheep, F1 of Dorset♂ × Small Tail Han sheep♀). The numbers of alleles for BM143,OarHH35,OarAE101,and BMS2508 are 9, 11, 14 and 9 in seven sheep populations, respectively. The polymorphism information content/number of effective alleles/ heterozygosity of BM143,OarHH35,OarAE101 and BMS2508 were 0.7073/3.7231/0.7314, 0.8267/6.4399/0.8447,0.5743/2.5178/0.6028,0.6172/3.0712/0.6744 in 286 sheep, respectively. The results revealed the greatest genetic variation at OarHH35 locus and the lowest at OarAE101, the greatest genetic variation in Small Tail Han sheep and the lowest in Hu sheep among seven sheep populations. In the unweighted pair group method with arithmetic mean (UPGMA) dendrograms based on Nei's DA distance and Nei's DS standard genetic distance, the Chinese native breeds (Small Tail Han sheep, Ujumqin sheep, Hu sheep) were grouped together, then with Charolais sheep. The F1 crossbred sheep, and the two British native sheep (Suffolk sheep, Dorset sheep) also clustered together. Microsatellite genotyping in sheep provided a useful tool for examining the genetic relationships among breeds(populations).     

新Y-STR DYS605在山西汉族人群中的多态性分布

为了应用更多的Y染色体特异性STR基因座以用于法医学和人类遗传学研究,本文用PCR结合PAGE技术检测128例山西汉族无关男性DYS605等位基因分布状况。结果显示：山西地区汉族男性DYS605基因座观察到22,21,20,19,18共5个等位基因,基因频率分别为0.0156;0.1797;0.4531;0.2891;0.0625。等位基因20和19之间的电泳距离在非变性胶上非常接近,要有足够的电泳距离才能区分。测序表明该基因座包括3个串联重复区,其中一个为可变重复区。20例女性DNA未发现扩增产物。Abstract: We study the polymorphism at DYS605 ,a new tetranucleotide Y-STR locus,in a Chinese Han population of Shanxi to meet the need of more genetic markers in forensic practice and genetic analysis. DNA were extracted from 128 unrelated male venous blood, and amplified using GDB primers. PCR products were detected using non-denaturing polyacrylamide gel electrophoresis and silver staining. Five alleles named 22,21,20,19,18 were observed with frequency of 0.0156;0.1797;0.4531;0.2891;0.0625. PCR products were not found in female DNA. Using a long enough gel for a long electrophoresis time is strongly encouraged because the rung between allele 20 and allele19 is smaller than expected. Allele sequences show that the repetitive units of DYS605 were composed with the variant units and non-variant units.     

维生素C葡萄糖苷的研究现状及发展前景

维生素C葡萄糖苷是卫生署公布认可的美白添加剂之一,利用糖基转移酶的特异性转糖基作用进行生物转化合成是目前维生素C葡萄糖苷的唯一生产途径。对维生素c葡萄糖苷的生产方法进行了讨论,着重阐述了其制备（生物转化合成法）、纯化及结晶三大生产工艺的国内外研究现状,指出国内实现维生素C葡萄糖苷工业化生产的发展方向。     

穿心莲内酯体外抗白念珠菌生物膜作用的初步研究

目的研究穿心莲内酯对体外白念珠菌生物膜的影响。方法采用XTT减低法评价穿心莲内酯对白念珠菌生物膜及其黏附性的影响;镜下观察该药对白念珠菌生物膜的形态学影响;细胞毒性试验检测该药的毒副作用。结果穿心莲内酯对白念珠菌生物膜的SMIC50、SMIC80分别是250、1000μg/ml;1000μg/ml及100μg/ml时对白念珠菌的早期黏附及菌丝生长有抑制作用;对人细胞毒性较弱。结论穿心莲内酯对体外白念珠菌生物膜有显著的抑制作用。     

拟南芥生长素受体基因TIR1启动子的克隆及序列分析

以野生型拟南芥 (Arabidopsis Columbia)基因组DNA为模板,通过PCR扩增得到拟南芥生长素受体基因TIR1启动子2008片段,将该片断克隆到PGM-T载体上.序列分析表明,该启动子大小为2008bp,RNA聚合酶识别序列TATA-box,TIR1特异表达和增强序列CAAT-box皆完整,与已报道的序列比较仅有3个核苷酸发生改变,同源性为99.85%.将该启动子与GUS基因融合,构建成表达载体后,在拟南芥和烟草叶片中做瞬时表达,结果分析显示:拟南芥和烟草叶片中均有GUS 酶活性存在.     

贵州小型猪肠菌群失调模型的制备

目的 探索小型猪肠菌群失调模型的制备方法.方法 分别采用大黄和氨苄青霉素灌服,通过检测贵州小型猪新鲜粪便中双歧杆菌、乳酸杆菌、肠杆菌、肠球菌的数量,了解其肠菌群变化情况.结果 两种方法都可导致贵州小型猪肠菌群改变.两组相比,大黄组菌群变化速度较快、持续时间较短;氨苄青霉素组菌群变化速度较慢、持续时间较长.结论 两种方法均可制备比较稳定的贵州小型猪肠菌群失调模型,但具体表现不尽相同,应用时可根据需要选择.